Tuesday, November 29, 2011
Tuesday, November 15, 2011
The extraction process-halfway through Day One
I will test my hypothesis using data collected in the field at RMBL in the Rocky Mountains of Colorado. I have spent the last seven weeks utilizing this data by collecting glucocorticoid levels from fecal samples in a precise three day extraction process. As seen in the picture above, the tubes contain a yellowish liquid that is a solution of the hormone, alcohol, and water. This mixture evaporates overnight in the vacuum centrifuge, as pictured in the photo below, so that the end result will only be the reconstituted hormone. The entire procedure involves three strict twenty-four hour sessions where I would repeatedly centrifuge and bathe the samples in a hot water bath, put them in a vacuum centrifuge, vortex, and then place them in the -20 degree freezer. The end result was then pipetted into exact sample sizes, as exemplified in the first picture at the top of the page, and shipped off for analysis.
Now that the extractions have been completed, the next step is to analyze blood smears for various neutrophils, leukocytes, monocytes, eosinophils, and any obvious parasites. This work is done using a microscope and will be measured by counting the number of white blood cells until we have one hundred total in each one of our samples. This portion of my project is especially important because it is the main indicator of health and will be used for comparisons with the glucocorticoid levels as well as the testosterone levels.
Once the blood smears have been examined I will begin the testosterone assays, which will reveal the relative levels of testosterone in each individual marmot. This will be the final component of my project and hopefully will reveal any connection between dominance and survival in regards to parasite and white blood cell count.